Multi-drug-resistant Gram-negative rod-shaped bacteria (MRGN) have become increasingly widespread in recent years as a cause of nosocomial infections worldwide. Antibiotics used for standard therapy are largely ineffective in the case of MRGN infections. This is why infections with multi-drug-resistant pathogens have to be identified very early on in order to initiate the right therapy with one of the few reserve antibiotics still available as soon as possible. MRGN bacteria can also survive on inanimate surfaces and spread via contaminated objects. Early detection is important so as to be able to take special hygienic measures and prevent these problematic pathogens from spreading. A Fraunhofer EMFT research team is collaborating with the SME GBN Systems GmbH, the Institute of Microbiology and Hygiene at Regensburg University Hospital and Asklepios Kliniken GmbH to develop a compact, comprehensive system that will enable rapid on-site detection of MRGN bacteria.